Strain Information | |
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Image | |
BRC No. | RBRC05447 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(ACTB-tTS)1Mllo |
Former Common name | Actin-tTS Tg, B6-ACTB-tTS Tg |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Moises Mallo, Max-Plank Institute of Immunobiology. C57BL/6 and 129 mixed background. |
Strain description | ACTB-tTS transgenic mice. These mice express tetracycline-controlled transcriptional silencer (tTS) gene under the control of the human beta actin promoter. By the combination with homozygous tetO-modified allele, expression from the gene can be blocked in the absence of doxycycline and restore when the antibiotic is administered to the animal. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Nat. Commun., 12(1) 5146 (2021). 34446732 Biol. Psychiatry, 67, 770-773 (2010). 20163789 Genomics, 81, 356-360 (2003). 12676559 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tTSE. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNAUNtTStransgene insertion 1, Moises Mallohuman beta-actin promoter, E. coli Tetracycline repressor (tetR), human Zfp KRAB domain Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Ordering Information | |
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Donor DNA | human beta actin promoter, tTS (E. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNA), chicken globin genomic DNA, SV40 polyA signal |
Research application | Tet system |
Specific Term and Conditions | The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEVELOPER (Dr. Moises Mallo, (Max-Plank Institute of Immunobiology) is requested. Genomics, 81(4):356-360 2003. The RECIPIENT must contact the DEVELOPER in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEVELOPER and must obtain a prior written consent from the DEVELOPER. |
Depositor | Kenji Tanaka (Keio University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |