Strain Data Sheet

RBRC05452

Strain Information

Image
BRC No.RBRC05452
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nlgn3<tm1.1(tetO)Rhn>
Former Common nameNlgn3 tetO KI, B6-Nlgn3/tetO KI
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Kenji Tanaka, Columbia University in 2007. CSL3 ES cells derived from 129S6/SvEvTac were used. FRT-flanked neo selection cassette was removed by crossing with ROSA-Flpe mice. The mutant mice were backcrossed to 129S6 over 5 times. C57BL/6 and 129 mixed background.
Strain descriptionNlgn3-tetO knock-in mice. tetO was inserted just upstream of the Nlgn3 gene. Expression pattern of the mutant allele is the same as that seen in wild-type mice. By the combination with tTA and tTS, tTA-mediated overexpression and tTS-mediated knockout can be generated respectively in the absence of doxycycline and restore when restore when the antibiotic is administered to the animal.
Colony maintenanceHomozygote x Hemizygote [or Crossing to C57BL/6JJcl]
ReferencesBiol. Psychiatry, 67, 770-773 (2010). 20163789

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nlgn3neuroligin 3XNlgn3<tm1.1Rhn>targeted mutation 1.1, Rene Hen

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tetOtetracycline operator (E. coli)XtetO

Ordering Information

Donor DNAP1 Phage loxP sites, yeast FRT (flipase recombination target) site, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, mouse Nlgn3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Psychiatry, 67, 770-773 (2010).
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data