Strain Information | |
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Image | |
BRC No. | RBRC05454 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129B6(Cg)-Actb<tm1(tetO-ChR2*C128S/EYFP)Kftnk> |
Former Common name | tetO-ChR2 KI, B6-tetO-ChR2/YFP KI |
H-2 Haplotype | |
ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
Background strain | |
Appearance | |
Strain development | Developed by Kenji Tanaka and Akihiro Yamanaka, National Institute for Physiological Sciences. (C57BL/6 x 129) F1 ES cells were used. FRT-flanked neo selection cassette was removed by crossing with ROSA-Flpe mice. C57BL/6 and 129 mixed background. |
Strain description | The tetO-ChR2 (C128S) cassette was inserted just downstream of the polyadenylation signal of Actb gene. Homozygous mutant mice are embryonic lethal. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Nat. Neurosci., 22(5):770-777 (2019). 30988523 Cell Rep., 36(4):109427(2021). 34320360 Cell Rep., 2(2):397-406 (2012). 22854021 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ChR2channelrhodopsin-2 (Chlamydomonas reinhardtii)5ChR2E. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)5EYFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal5 |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, Chlamydomonas Channelrhodopsin-2, jellyfish EYFP cDNA, SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse beta actin genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Cell Rep., 2(2):397-406 (2012). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper. |
Depositor | Kenji Tanaka (Keio University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Apr 2013 |
BRC mice in Publications |
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Abe Y, Kwon S, Oishi M, Unekawa M, Takata N, Seki F, Koyama R, Abe M, Sakimura K, Masamoto K, Tomita Y, Okano H, Mushiake H, Tanaka KF. Optical manipulation of local cerebral blood flow in the deep brain of freely moving mice. Cell Rep 36(4) 109427(2021) 34320360 Oishi M, Passlick S, Yamazaki Y, Unekawa M, Adachi R, Yamada M, Imayoshi I, Abe Y, Steinhäuser C, Tanaka KF. Separate optogenetic manipulation of Nerve/glial antigen 2 (NG2) glia and mural cells using the NG2 promoter. Glia 71(2) 317-333(2023) 36165697 Kato T, Mitsukura Y, Yoshida K, Mimura M, Takata N, Tanaka KF. Oscillatory population-level activity of dorsal raphe serotonergic neurons is inscribed in sleep structure. J Neurosci 42(38) 7244-55(2022) 35970565 Yamazaki Y, Abe Y, Fujii S, Tanaka KF. Oligodendrocytic Na+-K+-Cl- co-transporter 1 activity facilitates axonal conduction and restores plasticity in the adult mouse brain. Nat Commun 12(1) 5146(2021) 34446732 Kono A, Shikano Y, Tanaka KF, Yamaura K, Tsutsui-Kimura I. Inhibition of the dorsomedial striatal direct pathway is essential for the execution of action sequences. Neuropsychopharmacol Rep 43(3) 414-424(2023) 37553985 Murata J, Unekawa M, Kudo Y, Kotani M, Kanno I, Izawa Y, Tomita Y, Tanaka KF, Nakahara J, Masamoto K. Acceleration of the Development of Microcirculation Embolism in the Brain due to Capillary Narrowing. Stroke 54(8) 2135-2144(2023) 37309687 |