Strain Data Sheet

RBRC05474

Strain Information

Image
BRC No.RBRC05474
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Foxj1-Cluap1,lacZ)14Hmd
Former Common nameFxJ1-1.1 Qilin-lacZ (14), B6-Foxj1-Qilin/LacZ Tg#14
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. 1.1 kb segment containing human FoxJ1 promoter and Qilin cDNA-IRES LacZ genes were injected into the pronuclei of ((C57BL/6 x BALB/c)F1 x B6129-Qilin<tm1.1Hmd>)F1 fertilized eggs. C57BL/6 and 129 mixed background.
Strain descriptionTransgene specifically express Cluap1 (Qilin) and LacZ reporter in pit cells of embryonic node. Tg expression can be monitored by X-gal staining.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesDev. Biol., 381(1), 203-212 (2013). 23742838

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cluap1clusterin associated protein 1UNCluap1Qilinhuman Foxj1 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UN

Ordering Information

Donor DNAHuman Foxj1 promoter, Mouse Qilin gene cDNA, Virus IRES sequence, E. coli LacZ gene
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 381(1), 203-212 (2013).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data