Strain Data Sheet

RBRC05476

Strain Information

Image
BRC No.RBRC05476
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3(129)-Tg(NDE-hsp-Kif3a,lacZ)1Hmd
Former Common nameNDE-Kif3a-lacZ (1), B6-NDE-Kif3a/LacZ Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Satoko Yoshiba and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. The NDE-hsp-Kif3a,lacZ transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6, 129 and C3H mixed background.
Strain descriptionThis transgenic mouse expresses Kif3a and lacZ genes on both sides of the node under the control of the mouse node-specific enhancer.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesScience, 338, 226-231 (2012). 22983710

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kif3akinesin family member 3AUNKif3amouse Nodal node-notochord enhancer, mouse Hsp68 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Ordering Information

Donor DNAmouse Kif3a gene cDNA, mouse node-notochord enhancer, mouse Hsp68 promoter, E. coli LacZ, SV40 polyA signal, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires)
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 338, 226-231 (2012).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data