Strain Data Sheet

RBRC05479

Strain Information

Image
BRC No.RBRC05479
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameD2.Cg-Gt(ROSA)26Sor
Former Common nameD2-ROSA26
H-2 Haplotype
ES Cell lineAB1 [129S7/SvEvBrd-Hprt<+>]
Background strain
Appearance
Strain developmentGt(ROSA)26Sor allele was generated by Philippe Soriano. This congenic strain was generated by crossing to DBA/2 from B6.129S7-Gt(ROSA)26Sor/J (JAX).
Strain descriptionDBA/2 congenic mice of Gt(ROSA)26Sor. lacZ expression is observed in all tissues of the embryos and in most tissues of adult mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to DBA/2CrSlc]
ReferencesProc. Natl. Acad. Sci. USA, 94, 3789-3794 (1997). 9108056

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor (adenovirus)6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6

Ordering Information

Donor DNAE. coli Neo-resistance gene, E. coli Beta-galactosidase, retrovirus LTR, adenovirus major late transcript splice acceptor (SA) sequence, bovine growth hormone polyA, mouse ROSA26 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Gt(ROSA)26Sor line (Dr. Philippe Soriano, Mount Sinai School of Medicine). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorTakashi Shinohara (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data