Strain Information | |
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Image | |
BRC No. | RBRC05501 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | 129-Tmem119<tm1Tfur> |
Former Common name | 129-Obif KO, 129-Obif<tm1Tfur> |
H-2 Haplotype | |
ES Cell line | TC1 [129S6/SvEvTac] |
Background strain | |
Appearance | |
Strain development | Developed by Takahisa Furukawa, Osaka Bioscience Institute in 2006. 129 background. |
Strain description | Obif, osteoblast induction factor gene knockout mice. Exon 2 of Obif gene was replaced with PGK-neo cassette. Homozygous mutant mice show lower bone mass than controls at 14 days and 8 weeks old. |
Colony maintenance | Homozygote x Heterozygote [or Crossing to 129S6/SvEvTac] |
References | BMC Dev. Biol., 9, 70 (2009). 20025746 Dev. Growth Differ., 54(4):474-480 (2012). 22416756 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal5 |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 Poly A signal, mouse Tmem119 genomic DNA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Growth Differ., 54(4):474-480 (2012).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). |
Depositor | Takahisa Furukawa (Osaka Bioscience Institute) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |