Strain Information | |
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Image | |
BRC No. | RBRC05515 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129S4-Ern1<tm2.1Tiw> |
Former Common name | IRE1alpha floxed, B6-Ern1/dNeo floxed |
H-2 Haplotype | |
ES Cell line | RF8 [129S4/SvJae] |
Background strain | |
Appearance | |
Strain development | Developed by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. RF8 (129S4 derived) ES cells were used. Frt flanked neo cassette was removed by crossing with FLP mice. C57BL/6 and 129 mixed background. |
Strain description | IRE1alpha (Ern1) gene floxed mice. Exons 20 and 21 were flanked by loxP sites. Conditional IRE1alpha deficient mice can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). 19805353 |
Health Report | |
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Examination Date / Room / Rack | 2024/02/26Room:4-ARack:I 2024/02/19Room:4-ARack:I 2023/11/20Room:4-ARack:I 2023/09/22Room:4-ARack:I 2023/08/21Room:4-ARack:I 2023/05/22Room:4-ARack:I 2023/02/20Room:4-ARack:I 2022/11/21Room:4-ARack:I 2022/08/22Room:4-ARack:I 2022/05/23Room:4-ARack:I |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ern1endoplasmic reticulum (ER) to nucleus signaling 111Ern1<tm2.1Tiw>targeted mutation 2.1, Takao Iwawaki Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Ern1 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009).The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki). |
Depositor | Takao Iwawaki (Gunma University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |