Strain Information | |
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Image | |
BRC No. | RBRC05555 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;B6129-Ezh2<tm1Yugo>/Hko |
Former Common name | B6-Ezh2 floxed |
H-2 Haplotype | |
ES Cell line | M1 [(C57BL/6 x 129)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Haruhiko Koseki and Jun Shinga, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J. |
Strain description | Ezh2 floxed mice. A floxed neo cassette was inserted upstream of exon 18 and an additional loxP was inserted downstream of exon 19 of Ezh2 gene. Conditional Ezh2 deficient mice can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Neuron, 63, 600-613 (2009). 19755104 Blood, 118, 6553-6561 (2011). 22042701 |
Health Report | |
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Examination Date / Room / Rack | 2024/02/19Room:4-ARack:F 2023/11/20Room:4-ARack:F 2023/08/28Room:4-ARack:F 2023/08/21Room:4-ARack:F 2023/06/19Room:4-ARack:F 2023/05/22Room:4-ARack:F 2023/02/20Room:4-ARack:F 2022/11/21Room:4-ARack:F 2022/09/05Room:4-ARack:F 2022/08/22Room:4-ARack:F 2022/05/23Room:4-ARack:F |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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Donor DNA | phage P1 LoxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli Neomycin resistance gene, mouse Ezh2 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Haruhiko Koseki (RIKEN) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Ohka F, Shinjo K, Deguchi S, Matsui Y, Okuno Y, Katsushima K, Suzuki M, Kato A, Ogiso N, Yamamichi A, Aoki K, Suzuki H, Sato S, Arul Rayan N, Prabhakar S, Göke J, Shimamura T, Maruyama R, Takahashi S, Suzumura A, Kimura H, Wakabayashi T, Zong H, Natsume A, Kondo Y. Pathogenic Epigenetic Consequences of Genetic Alterations in IDH-Wild-Type Diffuse Astrocytic Gliomas. Cancer Res 79(19) 4814-4827(2019) 31431463 Fukui Y, Hirota Y, Aikawa S, Sakashita A, Shimizu-Hirota R, Takeda N, Ishizawa C, Iida R, Kaku T, Hirata T, Hiraoka T, Akaeda S, Matsuo M, Osuga Y. The EZH2-PRC2-H3K27me3 axis governs the endometrial cell cycle and differentiation for blastocyst invasion. Cell Death Dis 14(5) 320(2023) 37198149 |