Strain Data Sheet

RBRC05572

Strain Information

Image
BRC No.RBRC05572
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Rnf2<tm2Hko>
Former Common nameB6-Rnf2 floxed Ring1B cond
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN Center for Allergy and Immunology, and Miguel Vidal, Centro de Investigaciones Biologicas in 2002. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> were used. The mutant mice were crossed to C57BL/6J at least 5 times.
Strain descriptionRing1B (Rnf2) gene floxed mice. A loxP site was inserted upstream of Exon 2 including the ATG codon. A floxed neo cassette was inserted into intron 2.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesDev. Cell., 7, 663-676 (2004). 15525528

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rnf2ring finger protein 21Rnf2<tm2Hko>targeted mutation 2, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNABacteriophage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Rnf2 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data