Strain Information | |
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Image | |
BRC No. | RBRC05598 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129(Cg)-Vps41<tm1Ywd> |
Former Common name | mVam2 floxed, B6-Vps41 floxed |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University. An m Vam2-floxNeo allele, containing the FRT-loxP-Npt-FRT-loxP in intron 13, and the third loxP sequence in intron 17, was introduced into the mouse genome by R1 ES cell mediated gene replacement. The modified allele was converted to mVam2-floxed (Vps41<tm1Ywd>) by crossing with FLP mice (B6;SJL-Tg(ACTFLPe)9205Dym/J). The mutant mice were cross to C57BL/6. |
Strain description | mVam2 (Vps41) floxed mice. Exons 14 to 18 of the mVam2 gene were flanked by loxP sites. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] |
References | Dev. Cell, 22, 1163-1175 (2012). 22698281 Nucleic Acids Res., 33, e52 (2005). 15784610 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site13Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP13loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP13loxP |
Ordering Information | |
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Donor DNA | Escherichia coli Tn7 Right border and left border, Bacteriophage P1 loxP sites, Saccharomyces cerevisiae Frt site, Escherichia coli Tn5 Outer elements, mouse Vps41 genomic DNA |
Research application | Cre/loxP system FLP/frt system Neurobiology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University. |
Depositor | Yoh Wada (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |