Strain Information | |
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Image | |
BRC No. | RBRC05642 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Hspa5<tm1Aoe> |
Former Common name | Mutant BiP KI, B6-Hspa5 KI/HAtag |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Tomohiko Aoe, Chiba University Graduate School of Medicine in 2007. R1 ES cells were used. A floxed neo cassette was removed by crossing with CAG-Cre (B6) mice. The mutant mice were crossed to C57BL/6J. |
Strain description | BiP (Hspa5) gene knock-in mice. Knock-in mice expressing a HA-tag mutant BiP gene lacking the KDEL retrieval sequence. Homozygous mutant mice exhibit impaired surfactant secretion and die within a few hours after birth. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Mol. Cell. Biol., 28, 293-301 (2008). 17954555 Cell Death Differ., 14, 1475-1485 (2007). 17464327 Biochem. Biophys. Res. Commun., 366, 1048-1053 (2008). 18158912 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequence2HA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP |
Ordering Information | |
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Donor DNA | Mouse BiP gene, Human influenza hemagglutinin (HA) のHA tag 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3 |
Research application | Cre/loxP system Developmental Biology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Cell Death Differ., 14, 1475-1485 (2007). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR/DEVELOPER and notify him/her the research content. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE. |
Depositor | Tomohiko Aoe (Chiba University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |