Strain Information | |
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Image | |
BRC No. | RBRC05656 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Frem1<tm2.1Ksek> |
Former Common name | Qbrick<RGE>, B6-Frem1/RGE KI |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Kiyotoshi Sekiguchi, Institute for Protein Research, Osaka University in 2012. 129 derived ES cells were used. The mutant mice were crossed to C57BL/6J. A floxed neo cassette was removed by cre-mediated recombination. |
Strain description | Qbrick (Frem1) RGD (rg-Gly-Asp) knock-in mice. Exon 4 of Frem1 containing the codon encoding Asp207 was replaced with a mutated exon in which the codon encoding Asp207 was substituted with a codon encoding Glu207. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJmsSlc] |
References | Exp. Cell Res., 306, 9-23 (2005). 15878328 J. Cell Biol., 197, 677-689 (2012). 22613833 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frem1Fras1 related extracellular matrix protein 14Frem1<tm2.1Ksek>targeted mutation 2.1, Kiyotoshi Sekiguchi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP sequence |
Research application | Cell Biology Research Cre/loxP system |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Kiyotoshi Sekiguchi (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |