Strain Data Sheet

RBRC05656

Strain Information

Image
BRC No.RBRC05656
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Frem1<tm2.1Ksek>
Former Common nameQbrick<RGE>, B6-Frem1/RGE KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kiyotoshi Sekiguchi, Institute for Protein Research, Osaka University in 2012. 129 derived ES cells were used. The mutant mice were crossed to C57BL/6J. A floxed neo cassette was removed by cre-mediated recombination.
Strain descriptionQbrick (Frem1) RGD (rg-Gly-Asp) knock-in mice. Exon 4 of Frem1 containing the codon encoding Asp207 was replaced with a mutated exon in which the codon encoding Asp207 was substituted with a codon encoding Glu207.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesExp. Cell Res., 306, 9-23 (2005). 15878328
J. Cell Biol., 197, 677-689 (2012). 22613833

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frem1Fras1 related extracellular matrix protein 14Frem1<tm2.1Ksek>targeted mutation 2.1, Kiyotoshi Sekiguchi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Ordering Information

Donor DNABacteriophage P1 loxP sequence
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKiyotoshi Sekiguchi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data