Strain Data Sheet

RBRC05660

Strain Information

Image
BRC No.RBRC05660
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(CAG-CAT,spH)5Yyan
Former Common nameCATV-5, B6-CAG-CAT-SpH Tg#5, B6.D2-Tg (CAG-cat, -spH) 5Yyan
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yuchio Yanagawa, Gunma University Graduate School of Medicine and Hiromu Yawo, Tohoku University Graduate School of Life Sciences in 2002-2003. Transgene was injected into the introduced into the pronuclei of B6D2F1 fertilized eggs. The Tg mice were crossed to C57BL/6J.
Strain descriptionVesicular exocytosis at presynaptic terminals is fundamental function for neurotransmission and brain function. The lumen of a secretory vesicle is acidic (pH 5.6), whereas it becomes neutral (pH 7.4) instantaneously upon exocytosis. The intravesicular change of pH can be detected by a fluorescence change of a pH-sensitive derivative of green fluorescent protein (pHluorin). SynaptopHluorin (SpH) is a fusion protein of pHluorin and syntaxin (VAMP2) for targeting to secretory vesicles. CAG-CAT-SpH transgenic mouse expresses SpH under the control of CAG promoter by cre-mediated recombination. This strain is useful for studies of presynaptic terminals in various regions of the brain. Tg mice mice show no obvious abnormality.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
ReferencesGenesis 42, 53-60 (2005). 15880564

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Vamp2vesicle-associated membrane protein 2UNVamp2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
pHluorinpH-sensitive Green Fluorescent Protein (Aequorea victoria)UNpHluorin

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), P1 phage loxP sites, E. coli Chloramphenicol acetyltransferase (CAT), SV40 Small T antigen polyA, mouse vesicular membrane protein Vamp2 cDNA, Jellyfish pH-sensitive mutants GFP cDNA (pHluorin cDNA(Miesenbck, G., De Angelis, D.A., Rothman, J. E. Nature, 394, 192-195, 1998).)
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis 42, 53-60 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Any profit organizations cannot use the BIOLOGICAL RESOURCE without contracting license agreements with TOHOKU University.
DepositorHiromu Yawo (Tohoku University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data