Strain Data Sheet

RBRC05662

Strain Information

Image
BRC No.RBRC05662
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcp4<tm2.1(cre)>
Former Common namePcp4-cre, B6-Pcp4/Cre KI
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2008. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6).
Strain descriptionPcp4 cre knock-in mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pcp4Purkinje cell protein 416Pcp4 <tm2.1(cre)Mtto>targeted mutation 2.1, Toshitada Takemori

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)16GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase16cre

Ordering Information

Donor DNAphage P1 cre recombinase, yeast FRT site, bovine growth hormone polyA signal, mouse Pcp4 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Akiko Ishige is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorToshitada Takemori (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data