Strain Data Sheet

RBRC05665

Strain Information

Image
BRC No.RBRC05665
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rasgrp4<tm1.2>
Former Common nameRasGRP4 KO mouse, B6-RasGRP4 KO
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2007. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6). And then, Exons 5 and 6 were deleted by crossing with CAG-cre mice.
Strain descriptionRasGRP4 (Rasgrp4) gene knockout mice. Exons 5 and 6 were deleted, leading to out-of-frame products.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rasgrp4RAS guanyl releasing protein 47Rasgrp4 <tm1.2Mtto>targeted mutation 1.2, Toshitada Takemori

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Ordering Information

Donor DNAphage P1 loxP site, mouse RasGRP4 genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorToshitada Takemori (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data