Strain Information | |
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Image | |
BRC No. | RBRC05665 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Rasgrp4<tm1.2> |
Former Common name | RasGRP4 KO mouse, B6-RasGRP4 KO |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2007. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6). And then, Exons 5 and 6 were deleted by crossing with CAG-cre mice. |
Strain description | RasGRP4 (Rasgrp4) gene knockout mice. Exons 5 and 6 were deleted, leading to out-of-frame products. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP |
Ordering Information | |
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Donor DNA | phage P1 loxP site, mouse RasGRP4 genomic DNA |
Research application | Cre/loxP system Immunology and Inflammation Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Toshitada Takemori (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |