Strain Data Sheet

RBRC05701

Strain Information

Image
BRC No.RBRC05701
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(FLEXCAG-VSFP-Butterfly)552Bsi
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Thomas Knoepfel, RIKEN Brain Science Institute.
Strain descriptionMice carry the gene for the voltage sensor, VSFP-Butterfly flanked by loxP sites. Expression will be activate by cre recombinase following crossing with a cre driver mouse.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]
ReferencesJ. Neurophysiol., 108, 2323-2337 (2012). 22815406
Nat. Rev. Neurosci., 13, 687-700 (2012). 22931891

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ci-VSPCiona intestinalis voltage sensor-containing phosphataseUNCi-VSP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox2272phage P1 loxP variantUNlox2272

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox2272phage P1 loxP variantUNlox2272

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCitrineYellow Fluorescent Protein (Aequorea victoria)UNmCitrine

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mKateRed Fluorescent Protein (Entacmaea quadricolor)UNmKateCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Ordering Information

Donor DNAAequorea victoria Green Fluorescent Protein and derivatives coding sequence, Ciona intestinalis Ci-VSP, Entacmaea quadricolor Far-red Fluorescent Protein (mKate) and derivatives coding sequence, Bovine growth hormone polyadenylation (poly-A) signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE)
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorThomas Knöepfel (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form ( English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Contact Information

BRC mice in Publications

No Data