Strain Information | |
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Image | |
BRC No. | RBRC05701 |
Type | Transgene |
Species | Mus musculus |
Strain name | STOCK Tg(FLEXCAG-VSFP-Butterfly)552Bsi |
Former Common name | |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Thomas Knoepfel, RIKEN Brain Science Institute. |
Strain description | Mice carry the gene for the voltage sensor, VSFP-Butterfly flanked by loxP sites. Expression will be activate by cre recombinase following crossing with a cre driver mouse. |
Colony maintenance | Carrier x Carrier [or Crossing to C57BL/6JJcl] |
References | J. Neurophysiol., 108, 2323-2337 (2012). 22815406 Nat. Rev. Neurosci., 13, 687-700 (2012). 22931891 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ci-VSPCiona intestinalis voltage sensor-containing phosphataseUNCi-VSP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox2272phage P1 loxP variantUNlox2272 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox2272phage P1 loxP variantUNlox2272 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCitrineYellow Fluorescent Protein (Aequorea victoria)UNmCitrine Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mKateRed Fluorescent Protein (Entacmaea quadricolor)UNmKateCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Ordering Information | |
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Donor DNA | Aequorea victoria Green Fluorescent Protein and derivatives coding sequence, Ciona intestinalis Ci-VSP, Entacmaea quadricolor Far-red Fluorescent Protein (mKate) and derivatives coding sequence, Bovine growth hormone polyadenylation (poly-A) signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Thomas Knöepfel (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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No Data |