Strain Data Sheet

RBRC05708

Strain Information

Image
BRC No.RBRC05708
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Mstn<tm1.1Hko>/HkoRbrc
Former Common nameGdf8 delta lox/+ (GWAS002), B6-Gdf8 KO, B6.Cg-Gdf8<tm1.1Hko>
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Haruhiko Koseki, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129))F1-Kitl<+> were used for the generation of knockout mice. Exon 2 and neo cassette were removed by crossing with CAG-Cre mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain descriptionGdf8 (Mstn) gene knockout mice. Exon 2 was removed. Homozygous mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mstnmyostatin1Mstn<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Ordering Information

Donor DNAP1 Phage loxP site, Mouse Gdf8 (Mstn) genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data