Strain Data Sheet

RBRC05709

Strain Information

Image
BRC No.RBRC05709
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Klf9<tm1.1Hko>
Former Common nameKLF9 delta lox/+ (GWAS003), B6-Klf9 KO
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Haruhiko Koseki, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129))F1-Kitl<+> were used for the generation of knockout mice. Part of exon 1 and neo cassette were removed by crossing with CAG-Cre mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain descriptionKlf9 gene knockout mice. A part of exon 1 was removed. Homozygous mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Klf9Kruppel-like factor 919Klf9 <tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Ordering Information

Donor DNAP1 Phage loxP site, Mouse Klf9 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data