Strain Information | |
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Image | |
BRC No. | RBRC05716 |
Type | Targeted Mutation and Transgene |
Species | Mus musculus |
Strain name | B6D2-Pmis2<tm1Osb> Tg(Clgn-Spot3Flag)25Osb |
Former Common name | Pmis2-Flag Tg |
H-2 Haplotype | |
ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
Background strain | Jcl:B6D2F1 |
Appearance | |
Strain development | Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2005. |
Strain description | Testis specific Pmis2 (Flag-tag) gene transgenic mice. Endogenous Pmis2 expression was absent. Tg males are viable. |
Colony maintenance | KO : Homozygote x Homozygote ; Tg : Carrier x Carrier [or Crossing to Jcl:B6D2F1] |
References | Mol. Biol. Cell, 23, 2671-2679 (2012). 22621904 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pmis2PMIS2 transmembrane protein7Pmis2<tm1Oka>targeted mutation 1, Masaru Okabe1700071L20Rik, 4930479M11Rik Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7neo; neomycin; Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)UNFLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pmis2PMIS2 transmembrane proteinUNPmis21700071L20Rik, 4930479M11Rikmouse Clgn promoter |
Ordering Information | |
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Donor DNA | mouse Pmis2 genomic DNA, E. coli Neomycin resistance gene, mouse Calmegin promoter, FLAG tag (synthetic), mouse Pmis2 cDNA, rabbit polyA signal(以下残存の可能性有)Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal |
Research application | Developmental Biology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell, 23, 2671-2679 (2012).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masaru Okabe (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |