Strain Data Sheet
RBRC05722
Strain Information | |
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| Image | |
| BRC No. | RBRC05722 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Wbp2nl<tm1a(EUCOMM)Osb> |
| Former Common name | PAWP floxed |
| H-2 Haplotype | |
| ES Cell line | EGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Yuhkoh Satouh and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2011. |
| Strain description | Wbp2nl floxed mice. Exons 2 to 4 were flanked by loxP sites. |
| Colony maintenance | Homozygote x Homozygote [C57BL/6NJcl] |
| References | Biol Reprod. 2015 Oct;93(4):94. doi: 10.1095/biolreprod.115.131441. Epub 2015 Sep 16. 26377222 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Wbp2nlWBP2 N-terminal like15Wbp2nl<tm1a(EUCOMM)Osb>targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)15En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)15internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)15 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)15neo; neomycin; |
Ordering Information | |
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| Donor DNA | En2 splice acceptor (mouse En2 intron 2 / exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ, SV40 polyadenylation site, hBactP (human beta-actin promoter), E. coli neomycin phosphotransferase (neoR) gene, yeast FRT (flippase recombination target) sites, phage P1 loxP, mouse Wbp2nl genomic DNA |
| Research application | Cre/loxP system Developmental Biology Research FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Reprod. 2015 Oct;93(4):94. doi: 10.1095/biolreprod.115.131441. Epub 2015 Sep 16.Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. These strains were established by the DEPOSITOR by using knockout vectors of EUCOMM Consortium. The RECIPIENT shall acknowledge the EUCOMM Consortium as the source of the vectors of the strains. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masaru Okabe (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |