Strain Data Sheet

RBRC05743

Strain Information

Image
BRC No.RBRC05743
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pknox1<tm1.1Rygo>
Former Common namepKnox1 floxed, B6-Pknox1 floxed
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Dr. Ryo Goitsuka, Tokyo University of Science, Research Institute for Biomedical Sciences in 2008. Bruce 4 ES cells were used. The neo cassette was removed by crossing with FLP mice (B6). The mutant mice were crossed to C57BL/6 over 12 times.
Strain descriptionpKnox1 (Pknox1) gene floxed mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NJcl]
ReferencesPLoS One. 13(1):e0190702 (2018). 29293683

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pknox1Pbx/knotted 1 homeobox17Pknox1 <tm1Rygo>targeted mutation 1, Ryo Goitsuka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site17Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Ordering Information

Donor DNASaccharomyces cerevisiae FRT sequence, lambda bacteriophage P1 loxP sequence
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 13(1):e0190702 (2018).
DepositorRyo Goitsuka (Tokyo University of Science)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data