Strain Data Sheet

RBRC05751

Strain Information

Image
BRC No.RBRC05751
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ttll8<tm1Seto>
Former Common nameTTLL8 KO, B6-Ttll8 KO
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Koji Ikegami and Mitsutoshi Setou, Medical Photonics Research Center, Hamamatsu University School of Medicine in 2009. RENKA ES cells derived from C57BL/6N were used.
Strain descriptionTtll8 gene knockout mice. Exon1 and 3’UTR terminal region of nearby Mlc1 gene were deleted by homologous recombination.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesFEBS Lett., 583, 1957-1963 (2009). 19427864

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ttll8tubulin tyrosine ligase-like family, member 815Ttll8 <tm1Seto>targeted mutation 1, Mitsutoshi Setou

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAE. coli Neomycin resistance gene, HSV thymidine kinase promoter, HSV PolyA signal
Research applicationCell Biology Research
Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT must contact the DEPOSITOR prior to publishing the research results obtained by use of the BIOLOGICAL RESOURCE. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT must not distribute the BIOLOGICAL RESOURCE and the derivatives to third parties.
DepositorMitsutoshi Setou (Hamamatsu University School of Medicine)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data