Strain Data Sheet

RBRC05761

Strain Information

Image
BRC No.RBRC05761
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rftn1<tm1Ayos>
Former Common nameRaftlin KO, B6-Rftn1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Akihiko Yoshimura, Medical Institute of Bioregulation, Kyushu University.
Strain descriptionRaftlin (Rftn1) gene knockout mice. Exon 2 was replaced with a PGK-neo cassette. Homozygous mutant mice exhibit impaired T cell-mediated immune responses.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
ReferencesJ. Immunol., 182, 5929-5937 (2009). 19414744

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rftn1raftlin lipid raft linker 117Rftn1<tm1Ayos>targeted mutation 1, Akihiko Yoshimura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Mouse Rftn1 genomic DNA
Research applicationCell Biology Research
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 182, 5929-5937 (2009).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkihiko Yoshimura (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data