Strain Data Sheet

RBRC05841

Strain Information

Image
BRC No.RBRC05841
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(hOX-tTA)1Ahky
Former Common nameOrexin-tTA, B6-hOX-tTA Tg, C57BL/6-Tg(hOX-tTA)1Ahky
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2008. C57BL/6J background.
Strain descriptionOrexin neuron specific tTA transgenic mice.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]Tg homozygous and hemizygous mice are viable and fertile.
ReferencesSleep, 36(9):1391-404 (2013). 23997373
Neurosci. Res., 2012 Jul 31. pii: S0168-0102(12)00144-7. 22868039

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domainhuman orexin (hypocretin) promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Ordering Information

Donor DNAhuman orexin (hypocretin) promoter, E. coli Tet repressor (tetR), HSV VP16 activation domain, SV40 polyA
Research applicationNeurobiology Research
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data