Strain Information | |
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Image | |
BRC No. | RBRC05873 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Spata4<tm1a(KOMP)Osb>/3G |
Former Common name | Spata4 KO, B6-Spata4 floxed#3G |
H-2 Haplotype | |
ES Cell line | EGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb] |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Yoshitaka Fujihara and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2012. |
Strain description | Spata4 gene floxed mice (Knockout-first). |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NJcl] |
References | Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). 27357688 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Spata4spermatogenesis associated 48Spata4 <tm1a(KOMP)Osb>targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site8Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site8Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)8internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)8 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)8neo; neomycin; |
Ordering Information | |
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Donor DNA | En2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ, SV40 polyadenylation site, hBactP (human beta-actin promoter), E. coli neomycin phosphotransferase (neoR) gene, yeast FRT (flipase recombination target) sites, phage P1 loxP, mouse Spata4 genomic DNA 【クラス1】(以下「残存有」を交配にて取り除く)Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal 【クラス1】 |
Research application | Cre/loxP system Developmental Biology Research FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested. |
Depositor | Masaru Okabe (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |