Strain Information | |
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Image | |
BRC No. | RBRC05888 |
Type | Gene Trap |
Species | Mus musculus |
Strain name | C;129P2-Bri3bp<Gt(RST655)Byg>/Dkit |
Former Common name | BNAS1 gene trap mice |
H-2 Haplotype | |
ES Cell line | E14TG2aIV [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Daisuke Kitamura, Tokyo University of Science, Research Institute for Biomedical Sciences in 2005. RST655 gene trapped ES cells were used. The mutant mice were crossed to C57BL/6 or BALB/c over 6 times. BALB/c background (RBRC05888). |
Strain description | BNAS1 (Bri3bp) gene-trap mice. pGTOTMpfs gene- trap vector was inserted in the downstream of exon 1. |
Colony maintenance | Homozygote x Homozygote [or Crossing to BALB/cCrSlc] Homozygous mutant mice are viable and fertile. |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)5En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5neo; neomycin; |
Ordering Information | |
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Donor DNA | mouse Bri3bp gene, mouse Engrailed2 gene, E. coli beta-geo (beta-galactosidase-Neomycin-resistance fusion gene), SV40 Polyadenylation signal, E. coli pUC plasmid backbone, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human PLAP(placental alkaline phosphatase) cDNA, rat CD4 transmembrane domain (CD4TM) cDNA |
Research application | Cell Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. After deposition of the BIOLOGICAL RESOURCE, two years are assumed to be a collaborative research with the DEPOSITOR. |
Depositor | Daisuke Kitamura (Tokyo University of Science) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |