Strain Data Sheet

RBRC05899

Strain Information

Image
BRC No.RBRC05899
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-MacroH2A/EGFP)01Tois
Former Common nameMacoH2A-EGFP, B6-CAG-MacroH2A/GFP Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tomoko Nakanishi, Faculty of Medicine, Tottori University in 2009. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mutant mice were crossed to C57BL/6.
Strain descriptionMacroH2A-EGFP transgenic mice. This transgenic mice express the mouse MacroH2A1.2-EGFP under the control of a CAG promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrSlc]
ReferencesGenesis, 51(4):259-67 (2013). doi: 10.1002/dvg.22369. 23349035

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
MacroH2AMacroH2AUNMacroH2ACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Ordering Information

Donor DNACytomegalovirus Immediate-early enhancer, Chicken Beta actin promoter, Mouse MacroH2A1.2, Aequorea victoria Green fluorescent protein, Rabbit Beta globin polyA signal
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis. 2013 Jan 24. doi: 10.1002/dvg.22369.
DepositorTomoko Nakanishi (Tottori University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data