Strain Data Sheet

RBRC05923

Strain Information

Image
BRC No.RBRC05923
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Eed<tm1Homy>
Former Common nameEed cKO mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with FLPe mice.
Strain descriptionEed floxed mice. Exon 6 of the Eed gene was flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesLeukemia, 26, 2557-2560 (2012). 22733077

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Eedembryonic ectoderm development7Eed<tm1Homy>targeted mutation 1, Hiroaki Honda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Ordering Information

Donor DNAmouse Eed genomic DNA, yeast FRT (flipase recombination target) site, phage P1 loxP
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data