Strain Data Sheet

RBRC05945

Strain Information

Image
BRC No.RBRC05945
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Pax6<tm1Ued>
Former Common namePax6 cKO mice
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by David J. Price, The University of Edinburgh. E14TG2a ES cells were used. CD1(ICR) mixed background.
Strain descriptionPax6 floxed mie. Exons 5 and 6 were flanked by loxP sites. No brain phenotype known unless crossed with Cre deleter mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to Crlj:CD1(ICR)]
ReferencesNeural Dev., 4:19. doi: 10.1186/1749-8104-4-19 (2009). 19500363

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pax6paired box gene 62Pax6<tm1Ued>targeted mutation 1, University of Edinburgh

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAP1 phage loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neo resistance gene, mouse Pax6 genomic DNA
Research applicationCre/loxP system
Specific Term and Conditions1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Simpson, Ian, Thomas Pratt, John Mason and David J Price (2009) Normal ventral telencephalic expression of Pax6 is required for normal development of thalamocortical axons in embryonic mice. Neural Development 4:19. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) The RECIPIENT shall permit the DEPOSITOR to use the Results for research and education purposes only. 4) The RECIPIENT shall not make commercial use of any Results unless such commercial use is specifically permitted by the DEPOSITOR and governed by a further written agreement with the DEPOSITOR under which the DEPOSITOR shall be entitled to an equitable share of any revenue received by the RECIPIENT from such commercial use. 5) The RECIPIENT agrees to promptly notify the DEPOSITOR of any inventions made by the RECIPIENT in the performance of the Project. 6) The Material and any confidential information related to the BIOLOGICAL RESOURCE shall not be used for any commercial purpose or any commercially sponsored research whatsoever (whether such use is granted free of charge or not). 7) At least sixty (60) days prior to any public presentation or publication related to the purpose set out in clause 2(a), the RECIPIENT shall supply the DEPOSITOR with a copy of any such disclosure for review. 8) The DEPOSITOR shall have sixty (60) days from receipt of such manuscript to notify the RECIPIENT of any confidential information which is to be deleted, prior to publication.
DepositorDavid J. Price (The University of Edinburgh)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data