Strain Data Sheet

RBRC05952

Strain Information

Image
BRC No.RBRC05952
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CD2-Lrrc32,GFP)1Hfuj
Former Common nameGARP-Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hodaka Fujii, Research Institute for Microbial Diseases, Osaka University in 2009. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionCD2-GARP (Lrrc32) transgenic mice.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesJ. Immunol., 190, 5057-5064 (2013). 23576681

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lrrc32leucine rich repeat containing 32UNLrrc32human CD2 promoter, human CD2 polyA signal, human CD2 locus control region

Ordering Information

Donor DNAhuman CD2 promoter, moues GARP (LRRC32) cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), Aequorea victoria enhanced green fluorescent protein (GFP), human CD2 polyA signal, human CD2 locus control region
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 190, 5057-5064 (2013).
DepositorHodaka Fujii (Hirosaki University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data