Strain Data Sheet

RBRC05998

Strain Information

Image
BRC No.RBRC05998
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Nes-cre/ERT2)4Imayo
Former Common nameNestin-CreERT2 Line4 ; B6-Nes-CreER#4
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2005. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionNestin-CreER transgenic mice. CreERT2 is expressed by neural stem/progenitor cells. CreERT2-expression level is intermediate.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesGenesis. 2006 44(5):233-8. 16652364

Health Report

Examination Date / Room / Rack2024/02/19Room:4-ARack:H
2023/11/20Room:4-ARack:H
2023/08/21Room:4-ARack:H
2023/05/22Room:4-ARack:H
2023/02/20Room:4-ARack:H
2022/11/21Room:4-ARack:H
2022/08/22Room:4-ARack:H
2022/05/23Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signalUN

Ordering Information

Donor DNARat Nestin promoter, Nestin 2nd intron enhancer, SV40 PolyA signal, Cre recombinase (P1 pharge), human ER ligand binding domain (ERT2)
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis. 2006 44(5):233-8.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
DepositorDrs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Baba Y, Onishi-Sakamoto S, Ide K, Nishifuji K.
Nestin is a marker of unipotent embryonic and adult progenitors differentiating into an epithelial cell lineage of the hair follicles.
Sci Rep 12(1) 17820(2022) 36280775