Strain Information | |
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Image | |
BRC No. | RBRC05999 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6-Tg(Nes-cre/ERT2)5-1Imayo |
Former Common name | Nestin-CreERT2 Line5-1 ; B6-Nes-CreER#5-1 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2005. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. |
Strain description | Nestin-CreER transgenic mice. CreERT2 is expressed by neural stem/progenitor cells. CreERT2-expression level is low. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Genesis. 2006 44(5):233-8. 16652364 |
Health Report | |
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Examination Date / Room / Rack | 2024/02/19Room:4-ARack:L 2023/11/20Room:4-ARack:L 2023/08/21Room:4-ARack:L 2023/05/22Room:4-ARack:L 2023/02/20Room:4-ARack:L 2022/12/05Room:4-ARack:L 2022/11/21Room:4-ARack:L 2022/08/22Room:4-ARack:L 2022/05/23Room:4-ARack:L 2022/05/16Room:4-ARack:L |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinaseUNcrerat Nestin promoter, Nestin 2nd intron enhancer Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Ordering Information | |
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Donor DNA | Rat Nestin promoter, Nestin 2nd intron enhancer, SV40 PolyA signal, phage P1 Cre recombinase, human ER ligand binding domain |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis. 2006 44(5):233-8.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary). |
Depositor | Drs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Shigesada N, Shikada N, Shirai M, Toriyama M, Higashijima F, Kimura K, Kondo T, Bessho Y, Shinozuka T, Sasai N. Combination of blockade of endothelin signalling and compensation of IGF1 expression protects the retina from degeneration. Cell Mol Life Sci 81(1) 51(2024) 38252153 |