Strain Information | |
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Image | |
BRC No. | RBRC06040 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | ICR.Cg-Hcn4<tm1(tTA)>/1Kyo |
Former Common name | ICR-Tet-HCN4#1, ICR-Hcn4/tTA KI#1 |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Takahiro Ishii, Noriyuki Nakashima and Harunori Ohmori, Faculty of Medicine, Kyoto University. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 and ICR over 20 times, respectively. C57BL/6 background (line 1: RBRC06038, line 2: RBRC06039), ICR background (line 1: RBRC06040, line 2: RBRC06040). |
Strain description | Hcn4 tTA knock-in mice. |
Colony maintenance | Heterozygote x Wild-type [or Crossing to Slc:ICR] Homozygous mutant mice may be embryonic lethal. |
References | J. Physiol., 591, 1749-1769 (2013). 23318872 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hcn4hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1(tTA)Nnka>targeted mutation 1, Noriyuki NakashimaE. coli tet operator, cytomegalovirus CMV promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHhuman growth hormone gene9GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter URA3Orotidine-5'-phosphate (OMP) decarboxylase (yeast)9URA3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)9tTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal9 |
Ordering Information | |
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Donor DNA | tetracycline transactivator tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), SV40 polyA signal, Phage P1 loxP sites, Herpes simplex virus thymidine kinase promoter, E. coli neomycin resistance gene, Herpes simplex virus thymidine kinase polyA signal, yeast URA3 gene, human growth hormone polyA, tetO5-CMV (E. coli tet operator, cytomegalovirus CMV promoter), mouse Hcn4 genomic DNA |
Research application | Cre/loxP system Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Physiol., 591, 1749-1769 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER(S). |
Depositor | Harunori Ohmori (Kyoto University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |