Strain Data Sheet

RBRC06043

Strain Information

Image
BRC No.RBRC06043
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hes3<tm1Kag> Hes5<tm1Kag>
Former Common nameB6-Hes3 KO & Hes5 KO (LacZ knock-in) ; B6-Hes3/Hes5 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2000. TT2 ES cells were used. The mutant mice were crossed to C57BL/6 over 6 times.
Strain descriptionHes3 and Hes5 double knockout mice. The Hes3 gene was disrupted by a lac-neo cassette. The Hes5 gene was disrupted by IRES-nls-lacZ expression cassette.
Colony maintenanceHes3: Heterozygote x Wild-type [C57BL/6JJcl]; Hes5: Heterozygote x Wild-type [C57BL/6JJcl]
ReferencesDevelopment. 2004 131(22):5539-50. 15496443

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hes5hairy and enhancer of split 5 (Drosophila)4Hes5<tm1Kag>targeted mutation 1, Ryoichiro Kageyama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hes3hairy and enhancer of split 3 (Drosophila)4Hes3<tm1Kag>targeted mutation 1, Ryoichiro Kageyama

Ordering Information

Donor DNANeo (E. coli), EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES),beta-galactosidase (E. coli), mouse phosphoglycerate kinase promoter (PGK promoter), mouse Hes3 genomic DNA, Nuclear-localization signal from SV40 largeT antigen, Puromycin resistance gene (Streptomyces alboniger), mouse Hes5 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2004 131(22):5539-50.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
DepositorDrs. Itaru Imayoshi and Ryoichiro Kageyama (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data