Strain Data Sheet

RBRC06055

Strain Information

Image
BRC No.RBRC06055
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mc4r<tm1.1Hko>
Former Common nameMCR Delta lox/+ (GWAS023), B6-Mc4r KO
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of knockout mice. Exon 1 and neo cassette were removed by crossing with CAG-Cre mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain descriptionMCR4 (Mcr4) gene knockout mice. Exon 1 was removed.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack2023/04/10Room:3-BRack:G
2023/01/10Room:3-BRack:G
2022/10/11Room:3-BRack:G
2022/07/11Room:3-BRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mc4rmelanocortin 4 receptor18Mc4r<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Ordering Information

Donor DNAphage P1 loxP site, mouse Mc4r genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data