Strain Data Sheet

RBRC06146

Strain Information

Image
BRC No.RBRC06146
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB.Cg-Tg(HSA-Slk*K63R)3405
Former Common nameHSA K63R line 3405, FVB-HAS-SLK/K63R Tg#3405
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Luc Sabourin, Ottawa Hospital Research Institute. The transgene was injected into the pronuclei of (C57BL/6 x C3H)F1 fertilized eggs. The mutant mice were crossed to FVB/N for several times.
Strain descriptionTransgene expressing the HA-tagged, dominant negative cDNA (K63R) of SLK, under the control of the HAS promoter. Line 654 (RBRC06145) and line 3405 (RBRC06146). Tg mice exhibit some muscle defects. Line 654 and 3405 Tg mice have a high and low expression level of K63R, respectively. Line 654 Tg mice tend to yield smaller litters and fewer litters than their wild-type or line 3405 counterparts.
Colony maintenanceCarrier x Noncarrier [or Crossing to FVB/NJcl]
ReferencesSkelet Muscle., 3(1):16 (2013). 23815977

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slkmouse SLK mutated gene (upstream of full-length kinase-inactive SLK bearing a point mutation at lysine 63)UNSlkhuman skeletal actin promoter

Ordering Information

Donor DNAhuman skeletal actin promoter, mouse SLK mutated gene (upstream of full-length kinase-inactive SLK bearing a point mutation at lysine 63), HA (hemagglutinin) tag sequence
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Skelet Muscle., 3(1):16 (2013).
DepositorLuc Sabourin (Ottawa Hospital Research Institute)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data