Strain Data Sheet

RBRC06158

Strain Information

Image
BRC No.RBRC06158
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Del(10Slc5a4a-Slc5a4b)Csk
Former Common nameSGLT3a/3b double knockout mouse, SGLT3a/3b dKO, B6-10Slc5a4a/4b KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Chugai Pharmaceutical Co., Ltd. ES cells derived from C57BL/6N were used. C57BL/6N background.
Strain descriptionSlc5a4a (SGLT3a) and Slc5a4b (SGLT3b) genes double knockout mice. Exon 1 to 5 of the Slc5a4b and exon 1 to 2 and part of exon 3 of Slc5a4a were deleted. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-Type [or Crossing to C57BL/6NCrl]
References

Health Report

Examination Date / Room / Rack2024/01/09Room:3-BRack:J
2023/10/10Room:3-BRack:J
2023/07/10Room:3-BRack:J
2023/04/10Room:3-BRack:J
2023/01/10Room:3-BRack:J
2022/10/11Room:3-BRack:J
2022/07/11Room:3-BRack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc5a4bsolute carrier family 5 (neutral amino acid transporters, system A), member 4b10Slc5a4bdeletion, Chr 10, Chugai Pharmaceutical Co., Ltd.deletion, Chr 10, Chugai Pharmaceutical Co., Ltd.

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc5a4asolute carrier family 5, member 4a10Slc5a4adeletion, Chr 10, Chugai Pharmaceutical Co., Ltd.deletion, Chr 10, Chugai Pharmaceutical Co., Ltd.

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Ordering Information

Donor DNAphage P1 loxP site, mouse Slc5a4a genomic DNA, mouse Slc5a4b genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR. (Contact info: Mr. Taku Fukuzawa <fukuzawatk@chugai-pharm.co.jp>, Discovery Pharmacology Dept.)
DepositorKou-ichi Jishage (Chugai Pharmaceutical Co., Ltd.)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data