Strain Data Sheet

RBRC06159

Strain Information

Image
BRC No.RBRC06159
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Slc5a9<tm1Csk>
Former Common nameSGLT4 knockout mouse, SGLT4 KO, B6-Slc5a9 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Chugai Pharmaceutical Co., Ltd. ES cells derived from C57BL/6N were used to disrupt the Slc5a9 gene by homologous recombination. C57BL/6N background.
Strain descriptionSlc5a9 (SGLT4) gene knockout mice. Part of exon 2 through part of 4 was replaced with a PGK neo cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc5a9solute carrier family 5 (sodium/glucose cotransporter), member 94Slc5a9<tm1Csk>targeted mutation 1, Chugai Pharmaceutical Co., Ltd.

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Ordering Information

Donor DNAマウス Phosphoglycerate kinase-1遺伝子プロモーター, phage T7 gb2遺伝子プロモーター, 大腸菌 ネオマイシン耐性遺伝子 cDNA, 合成核酸 ポリA付加シグナル
Research application
Specific Term and ConditionsThe RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR. (Contact info: Mr. Taku Fukuzawa <fukuzawatk@chugai-pharm.co.jp>, Discovery Pharmacology Dept.)
DepositorKou-ichi Jishage (Chugai Pharmaceutical Co., Ltd.)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data