Strain Data Sheet

RBRC06160

Strain Information

Image
BRC No.RBRC06160
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Slc5a10<tm1Csk>
Former Common nameSGLT5 knockout mouse, SGLT5 KO, B6-Slc5a10 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Chugai Pharmaceutical Co., Ltd. ES cells derived from C57BL/6N were used to disrupt the Slc5a10 gene by homologous recombination. C57BL/6N background.
Strain descriptionSlc5a10 (SGLT5) gene knockout mice. Part of exon 3 through part of 6 was replaced with a PGK-neo cassette. Homozygous mutant mice exhibit abnormal renal reabsorption (increased urinary fructose and mannose excretion). Fatty liver is also observed in mice fed a high fructose diet. Viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesPLoS One. 2013;8(2):e56681. 23451068

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc5a10solute carrier family 5 (sodium/glucose cotransporter), member 1011Slc5a10<tm1Csk>targeted mutation 1, Chugai Pharmaceutical Co., Ltd.

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Ordering Information

Donor DNAマウス Phosphoglycerate kinase-1遺伝子プロモーター, phage T7 gb2遺伝子プロモーター, 大腸菌 ネオマイシン耐性遺伝子 cDNA, 合成核酸 ポリA付加シグナル
Research applicationMetabolism Research
Specific Term and ConditionsThe RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR. (Contact info: Mr. Taku Fukuzawa <fukuzawatk@chugai-pharm.co.jp>, Discovery Pharmacology Dept.)
DepositorKou-ichi Jishage (Chugai Pharmaceutical Co., Ltd.)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data