Strain Information | |
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Image | |
BRC No. | RBRC06161 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6J-Tg(3xARE/TK-NRF2/venus)F4Tiw |
Former Common name | OKD48-venus, B6-ARE-NRF2/venus Tg#F4 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Takao Iwawaki, Advanced Science Institute, RIKEN in 2010. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mice were backcrossed to C57BL/6J. |
Strain description | Keap1-dependent Oxidative stress Detector transgenic mice, named OKD48 (Keap1-dependent Oxidative stress Detector, No-48). The mice carry human Nrf2 and venus fusion gene under control of 3xARE promoter (oxidative stress inducible promoter). Only cells experiencing oxidative stress can be detected by venus. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Free Radic. Biol. Med., 72, 124-133 (2014). 24746614 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)UNFLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter NFE2l2nuclear factor, erythroid derived 2, like 2 (human)UNNFE2l2NRF23xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)UNVenus |
Ordering Information | |
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Donor DNA | 3xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter), human Nrf2(1-433) cDNA, Aequorea victoria venus (GFP) cDNA, FLAG tag, SV40 t intron, SV40 enhancer+ori, SV40 polyA signal, Polyomavirus enhancer+ori |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Free Radic. Biol. Med., 72, 124-133 (2014).The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Takao Iwawaki (Gunma University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |