Strain Data Sheet

RBRC06165

Strain Information

Image
BRC No.RBRC06165
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Edn3<tm1Ywa>
Former Common nameET3/129, B6-Edn3 KO
H-2 Haplotype
ES Cell lineJH1 [129S7/SvEvBrd]
Background strain
Appearance
Strain developmentDeveloped by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. JH1 ES cells were used to generate the mutant mice. 129 and C57BL/6 mixed background.
Strain descriptionET-3 (Edn3 / endothelin 3) gene knockout mice. A neomycin resistant cassette was inserted into the middle of exon 2. Homozygous mutant mice die after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
ReferencesCell, 79, 1277-1285 (1994). 8001160

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Edn3endothelin 32Edn3<tm1Ywa>targeted mutation 1, Masashi Yanagisawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)2GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2mouse RNA polymerase II promoter

Ordering Information

Donor DNAE. coli Neomycin resistance gene, mouse RNA polymerase II promoter, bovine growth hormone polyadenylation site, mouse Edn3 genomic DNA
Research applicationDevelopmental Biology Research
Hematological Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 79, 1277-1285 (1994).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
DepositorMasashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data