Strain Information | |
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Image | |
BRC No. | RBRC06197 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S4-Cckar<tm1Kym> |
Former Common name | CCKAR KO, B6-Cckar KO |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae] |
Background strain | |
Appearance | |
Strain development | Developed by Soichi Takiguchi and Akihiro Funakoshi, National Kyusu Cancer Center, Tetsuo Noda, The Cancer Institute, Japanese Foundation for Cancer Research and Kyoko Miyasaka, Tokyo Metropolitan Institute of Gerontology. J1 ES cells were used. The mutant mice were backcrossed to C57BL/6J. |
Strain description | Cckar gene knockout mice. Part of exon 1 and all of exon 2 were replaced with a NLS-lacZ neo cassette. Homozygous mutant mice are viable and fertile. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] |
References | Pancres, 24, 276-283 (2002). 11893936 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)5nls |
Ordering Information | |
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Donor DNA | SV40 Large T antigen nuclear localisation signal (NLS), E. coli Beta-Galactosidase gene (LacZ gene), Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Cckar genomic DNA |
Research application | Fluorescent Proteins/lacZ System Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Pancres, 24, 276-283 (2002). |
Depositor | Kyoko Miyasaka (Tokyo Kasei University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |