Strain Information | |
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Image | |
BRC No. | RBRC06203 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Pitx2<tm1.1Mbf> |
Former Common name | Pitx2 A-17 flox, B6-Pitx2 floxed |
H-2 Haplotype | |
ES Cell line | G4 [(129S6/SvEvTac x C57BL/6NCr)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Hidetaka Shiratori and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2001. G4 ES cells were used. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and 129 mixed background. |
Strain description | Pitx2 gene floxed mice. A loxP site was inserted in the intron 5, and a neo cassette with a 3’ loxP site was inserted downstream of intron 5. Homozygous mice are viable and fertile. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Dev. Dyn., 240, 195-203 (2011). 21089073 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pitx2paired-like homeodomain transcription factor 23Pitx2<tm1.1Mbf>targeted mutation 1.1, Milena B Furtado Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, mouse PGK1 promoter, E.coli neo gene, mouse PGK1 polyA signal sequence, mouse Pitx2 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Dyn., 240, 195-203 (2011). |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Live mice |
Strain Availability | Live mouse (more than 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |