Strain Information | |
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Image | |
BRC No. | RBRC06209 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Gt(ROSA)26Sor<tm1(Cerl2/3xHA)Hmd> |
Former Common name | ROSA26-3HA Dte KI, B6.Cg-Gt(ROSA)26Sor<tm1(Dte/3xHA)Hmd> |
H-2 Haplotype | |
ES Cell line | G4 [(129S6/SvEvTac x C57BL/6NCr)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Tetsuya Nakamura and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells were used. C57BL/6 and 129 mixed background. |
Strain description | Dte-3xHAtag ROSA26 conditional knock in mice. PGK-neo cassette with SV40 ployA and Dte (Dand5)-3xHA were inserted into the Gt(ROSA)26Sor. The Dte-3xHA expression can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Dev. Biol., 395(2):331-41 (2014). 25224222 Nat. Commun., 3:1322 (2012). doi: 10.1038/ncomms2319. 23271656 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Dte/3xHA)Hmd>targeted mutation 1, Hiroshi Hamada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Dand5DAN domain family, member 56Dand5Dte, Cerl2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequence6HA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 virus polyA signal sequence6 |
Ordering Information | |
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Donor DNA | Adenovirus splicing acceptor (SA), mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, SV40 virus polyA signal sequence, Influenza virus HA tag, phage P1 loxP sites, mouse Dand5 (Cerberus like 2) gene |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 3:1322 (2012). doi: 10.1038/ncomms2319. |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |