Strain Data Sheet

RBRC06209

Strain Information

Image
BRC No.RBRC06209
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm1(Cerl2/3xHA)Hmd>
Former Common nameROSA26-3HA Dte KI, B6.Cg-Gt(ROSA)26Sor<tm1(Dte/3xHA)Hmd>
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Tetsuya Nakamura and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells were used. C57BL/6 and 129 mixed background.
Strain descriptionDte-3xHAtag ROSA26 conditional knock in mice. PGK-neo cassette with SV40 ployA and Dte (Dand5)-3xHA were inserted into the Gt(ROSA)26Sor. The Dte-3xHA expression can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesDev. Biol., 395(2):331-41 (2014). 25224222
Nat. Commun., 3:1322 (2012). doi: 10.1038/ncomms2319. 23271656

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Dte/3xHA)Hmd>targeted mutation 1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dand5DAN domain family, member 56Dand5Dte, Cerl2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence6HA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor (adenovirus)6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 virus polyA signal sequence6

Ordering Information

Donor DNAAdenovirus splicing acceptor (SA), mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, SV40 virus polyA signal sequence, Influenza virus HA tag, phage P1 loxP sites, mouse Dand5 (Cerberus like 2) gene
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 3:1322 (2012). doi: 10.1038/ncomms2319.
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data