Strain Data Sheet

RBRC06219

Strain Information

Image
BRC No.RBRC06219
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Frmpd1<tm1Tfur>
Former Common nameFrmpd1, B6-Frmpd1 KO
H-2 Haplotype
ES Cell lineJM8 [C57BL/6N]
Background strainC57BL/6NTac
Appearance
Strain developmentDeveloped by Yoshihiro Omori and Takahisa Furukawa, Institute for Protein Research, Osaka University in 2012. JM8 ES cells were used.
Strain descriptionFrmpd1 gene knockout mice. Exons 3 and 4 were replaced with a PGK-neo cassette. Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NTac]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frmpd1FERM and PDZ domain containing 14Frmpd1<tm1Tfur>targeted mutation 1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4human PGK promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA4

Ordering Information

Donor DNAhuman PGK promoter, SV40 SV40 poly A signal, E. coli Neo
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data