Strain Information | |
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Image | |
BRC No. | RBRC06232 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S2-Prl<tm1Hmn> |
Former Common name | PRL-KO, B6-Prl KO |
H-2 Haplotype | |
ES Cell line | D3 [129S2/SvPas] |
Background strain | |
Appearance | |
Strain development | Developed by Nelson Horseman, University of Cincinnati and Minoru Tanaka, Mie University School of Medicine in 1997. D3 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 20 times. |
Strain description | Prl (prolaction) gene knockout mice. A PGK-neo cassette was inserted into the exon 4 of Prl gene. Homozygous mutant females are infertile due to an implantation defect. Homozygous mutant males are fertile. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6J(Crlj)] |
References | EMBO J., 16, 6926-6935 (1997). 9384572 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | E. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter, Mouse Prl genomic DNA |
Research application | Developmental Biology Research Immunology and Inflammation Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 16, 6926-6935 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | MInoru Tanaka (Nippon Veterinary and Animal Science University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |