Strain Data Sheet
RBRC06240
Strain Information | |
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| Image | |
| BRC No. | RBRC06240 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Hmgb1<tm1Ttg> |
| Former Common name | Hmgb1 cKO, B6-Hmgb1 floxed |
| H-2 Haplotype | |
| ES Cell line | E14.1 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Hideyuki Yanai and Tadatsugu Taniguchi, Institute of Industrial Science, University of Tokyo and Nobuaki Yoshida, Institute of Medical Science, University of Tokyo in 2011. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6. |
| Strain description | Hmgb1 gene floxed mice. Exons 2 to 4 were flanked by loxP sites. Homozygous mutant mice are viable, but show weak fertility. |
| Colony maintenance | Heterozygote x Wild-type [or Crossing to C57BL/6JJcl] |
| References | Proc. Natl. Acad. Sci. USA, 110, 20699-20704 (2013). 24302768 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP |
Ordering Information | |
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| Donor DNA | Phage P1 loxP sites, mouse Hmgb1 genomic DNA |
| Research application | Cre/loxP system Immunology and Inflammation Research |
| Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Tadatsugu Taniguchi (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jun 2014 |
BRC mice in Publications |
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Lu J, Yue Y, Xiong S. Extracellular HMGB1 augments macrophage inflammation by facilitating the endosomal accumulation of ALD-DNA via TLR2/4-mediated endocytosis. Biochim Biophys Acta Mol Basis Dis 1867(10) 166184(2021) 34087422 |