Strain Information | |
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Image | |
BRC No. | RBRC06251 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Ret<tm1Mat> Ptch1<tm1Mps> |
Former Common name | Ret YF; Ptch1 KO, B6-RetYF KI/Ptch1 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Ret Y1062F knock-in: Developed by Masahide Takahashi, Nagoya University Graduate School of Medicine. Ptch1 KO: Developed by Matthew Scott, Stanford University School of Medicine. |
Strain description | Ret Y1062F knock-in and Ptch1 KO double mutant mice. The Ret Y1062F knock-in allele consists of two amino acid replacement (KLY<1062> to KIF<1062>) within exon 19 and a floxed PGK-neo cassette inserted into intron 19. Part of exon 1 and all of exon 2 of Ptch1 gene were replaced with a lacZ-neo cassette in the Ptch1 KO allele. |
Colony maintenance | Ret: Heterozygote x Wild-type; Ptch1: Heterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc] |
References | Mol. Cell Biol., 24, 8026-8036 (2004). 15340065 Science, 277, 1109-1113 (1997). 9262482 Proc. Natl. Acad. Sci. USA, 107(29), 13051-13056 (2010). 20616061 |
Health Report | |
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Examination Date / Room / Rack | 2023/04/10Room:3-ARack:J 2023/01/10Room:3-ARack:J 2022/10/11Room:3-ARack:J 2022/07/11Room:3-ARack:J 2022/04/11Room:3-ARack:J |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)13 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)13 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | phage P1 LoxP sites, Mus musclus phosphoglycerate kinase promoter (PGK promoter), Escherichia coli Neomycin resistance gene, mouse Ret genomic DNA, Escherichia coli LacZ gene, mouse Ptch1 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Masashi Kato (Chubu University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |